|Application ||WB, E|
|Other Accession||NP_036066, 79068, 26383 (mouse), 291905 (rat)|
|Predicted||Mouse, Rat, Pig, Dog|
|Calculated MW||58282 Da|
|Other Names||Alpha-ketoglutarate-dependent dioxygenase FTO, 1.14.11.-, Fat mass and obesity-associated protein, FTO, KIAA1752|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-FTO (Mouse) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine (PubMed:18775698, PubMed:20376003). Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:22002720, PubMed:26458103). Has no activity towards 1- methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation (PubMed:18775698, PubMed:20376003). In particular, it is involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells (PubMed:26287746).|
|Cellular Location||Nucleus. Nucleus speckle|
|Tissue Location||Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.|
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Provided below are standard protocols that you may find useful for product applications.
FTO effect on energy demand versus food intake. Speakman JR. Nature, 2010 Apr 1. PMID 20360686.
Hypothalamic FTO is associated with the regulation of energy intake not feeding reward. Olszewski PK, et al. BMC Neurosci, 2009 Oct 27. PMID 19860904.
A mouse model for the metabolic effects of the human fat mass and obesity associated FTO gene. Church C, et al. PLoS Genet, 2009 Aug. PMID 19680540.
Loss-of-function mutation in the dioxygenase-encoding FTO gene causes severe growth retardation and multiple malformations. Boissel S, et al. Am J Hum Genet, 2009 Jul. PMID 19559399.
Inactivation of the Fto gene protects from obesity. Fischer J, et al. Nature, 2009 Apr 16. PMID 19234441.
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