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Goat Anti-Fumarase / FH Antibody
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, Pep-ELISA |
|---|---|
| Primary Accession | P07954 |
| Other Accession | NP_000134, 2271, 14194 (mouse), 24368 (rat) |
| Reactivity | Human |
| Predicted | Mouse, Rat, Dog |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 100ug/200ul |
| Isotype | IgG |
| Calculated MW | 54637 Da |
| Gene ID | 2271 |
|---|---|
| Other Names | Fumarate hydratase, mitochondrial, Fumarase, 4.2.1.2, FH |
| Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Goat Anti-Fumarase / FH Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | FH {ECO:0000303|PubMed:27037871, ECO:0000312|HGNC:HGNC:3700} |
|---|---|
| Function | Catalyzes the reversible stereospecific interconversion of fumarate to L-malate (PubMed:30761759). Experiments in other species have demonstrated that specific isoforms of this protein act in defined pathways and favor one direction over the other (Probable). |
| Cellular Location | [Isoform Mitochondrial]: Mitochondrion |
| Tissue Location | Expressed in red blood cells; underexpressed in red blood cells (cytoplasm) of patients with hereditary non-spherocytic hemolytic anemia of unknown etiology. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.
References
An approach based on a genome-wide association study reveals candidate loci for narcolepsy. Shimada M, et al. Hum Genet, 2010 Oct. PMID 20677014.
Fumarase: a mitochondrial metabolic enzyme and a cytosolic/nuclear component of the DNA damage response. Yogev O, et al. PLoS Biol, 2010 Mar 9. PMID 20231875.
UOK 262 cell line, fumarate hydratase deficient (FH-/FH-) hereditary leiomyomatosis renal cell carcinoma: in vitro and in vivo model of an aberrant energy metabolic pathway in human cancer. Yang Y, et al. Cancer Genet Cytogenet, 2010 Jan 1. PMID 19963135.
Follow-up examination of linkage and association to chromosome 1q43 in multiple sclerosis. McCauley JL, et al. Genes Immun, 2009 Oct. PMID 19626040.
Novel role of fumarate metabolism in dahl-salt sensitive hypertension. Tian Z, et al. Hypertension, 2009 Aug. PMID 19546378.
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