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Goat Anti-GRAF / ARHGAP26 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-GRAF / ARHGAP26 Antibody AF1503a
    AF1503a (0.3 µg/ml) staining of Human Heart lysate (35 µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UNA1
Other Accession NP_001129080, 23092, 71302 (mouse)
Reactivity Human
Predicted Mouse, Rat, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 92235 Da
Additional Information
Gene ID 23092
Other Names Rho GTPase-activating protein 26, GTPase regulator associated with focal adhesion kinase, Oligophrenin-1-like protein, Rho-type GTPase-activating protein 26, ARHGAP26, GRAF, KIAA0621, OPHN1L
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-GRAF / ARHGAP26 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms GRAF, KIAA0621, OPHN1L
Function GTPase-activating protein for RHOA and CDC42.
Cellular Location Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Note=Colocalizes with actin stress fibers and cortical actin structures.
Research Areas
Citations (0)

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Interaction of a cell with the extracellular matrix triggers integrin cell surface receptors to begin signaling cascades that regulate the organization of the actin-cytoskeleton. One of the proteins involved in these cascades is focal adhesion kinase. The protein encoded by this gene is a GTPase activating protein that binds to focal adhesion kinase and mediates the activity of the GTP binding proteins RhoA and Cdc42. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). Two transcript variants encoding different isoforms have been found for this gene.


Decreased expression of GRAF1/OPHN-1-L in the X-linked alpha thalassemia mental retardation syndrome. Barresi V, et al. BMC Med Genomics, 2010 Jul 6. PMID 20602808.
[Quantification of GRAF gene expression in patients with acute myeloid leukemia using EvaGreen real time quantitative PCR] Qian Z, et al. Zhonghua Yi Xue Yi Chuan Xue Za Zhi, 2010 Jun. PMID 20533268.
Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
Aberrant methylation of GTPase regulator associated with the focal adhesion kinase (GRAF) promoter is an adverse prognostic factor in myelodysplastic syndrome. Qian J, et al. Eur J Haematol, 2010 Aug. PMID 20374274.
Dissecting the thermodynamics of GAP-RhoA interactions. Jelen F, et al. J Struct Biol, 2009 Jan. PMID 18929667.

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$ 325.00
Cat# AF1503a
(40 western blots)
Availability: 7-10 days
Bulk Size
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