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Goat Anti-GRAP Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-GRAP Antibody AF1505a
    AF1505a (0.3 µg/ml) staining of human thymus lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13588
Other Accession NP_006604, 10750, 71520 (mouse)
Reactivity Human
Predicted Mouse, Rat, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 25337 Da
Additional Information
Gene ID 10750
Other Names GRB2-related adapter protein, GRAP
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-GRAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway.
Research Areas
Citations (0)

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This gene encodes a member of the GRB2/Sem5/Drk family. This member functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain.


Quantitative mass spectrometry of diabetic kidney tubules identifies GRAP as a novel regulator of TGF-beta signaling. Cummins TD, et al. Biochim Biophys Acta, 2010 Apr. PMID 19836472.
CD28 and Grb-2, relative to Gads or Grap, preferentially co-operate with Vav1 in the activation of NFAT/AP-1 transcription. Schneider H, et al. Biochem Biophys Res Commun, 2008 May 2. PMID 18295596.
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.
Detection of Grb-2-related adaptor protein gene (GRAP) and peptide molecule in salivary glands of MRL/lpr mice and patients with Sj枚gren's syndrome. Shiraiwa H, et al. J Int Med Res, 2004 May-Jun. PMID 15174222.
GC-GAP, a Rho family GTPase-activating protein that interacts with signaling adapters Gab1 and Gab2. Zhao C, et al. J Biol Chem, 2003 Sep 5. PMID 12819203.

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$ 325.00
Cat# AF1505a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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