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Goat Anti-GTRAP3-18 / JWA Antibody

Peptide-affinity purified goat antibody

     
  • WB - Goat Anti-GTRAP3-18 / JWA Antibody AF1515a
    AF1515a staining (0.1 µg/ml) of Human Brain lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
    detail
  • IHC - Goat Anti-GTRAP3-18 / JWA Antibody AF1515a
    AF1515a (3.75 µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • IHC - Goat Anti-GTRAP3-18 / JWA Antibody AF1515a
    AF1515a (3.75 µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • WB - Goat Anti-GTRAP3-18 / JWA Antibody AF1515a
    EB05631 (1ug/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer) . Detected by chemiluminescence.
    detail
  • WB - Goat Anti-GTRAP3-18 / JWA Antibody AF1515a
    EB05631 (1ug/ml) staining of K562 cell lysate (35µg protein in RIPA buffer) . Detected by chemiluminescence.
    detail
  • IHC - Goat Anti-GTRAP3-18 / JWA Antibody AF1515a
    EB05631 (3.75µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • IF - Goat Anti-GTRAP3-18 / JWA Antibody AF1515a
    EB05631 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and ER/cytoplasmic staining. The
    detail
  • FC - Goat Anti-GTRAP3-18 / JWA Antibody AF1515a
    EB05631 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
    detail
  • IHC - Goat Anti-GTRAP3-18 / JWA Antibody AF1515a
    EB05631 (3.75µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, FC, Pep-ELISA
Primary Accession O75915
Other Accession NP_006398, 10550
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 21615 Da
Additional Information
Gene ID 10550
Other Names PRA1 family protein 3, ADP-ribosylation factor-like protein 6-interacting protein 5, ARL-6-interacting protein 5, Aip-5, Cytoskeleton-related vitamin A-responsive protein, Dermal papilla-derived protein 11, GTRAP3-18, Glutamate transporter EAAC1-interacting protein, JM5, Prenylated Rab acceptor protein 2, Protein JWa, Putative MAPK-activating protein PM27, ARL6IP5, DERP11, JWA, PRA2, PRAF3
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-GTRAP3-18 / JWA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARL6IP5
Synonyms DERP11, JWA, PRA2, PRAF3
Function Regulates intracellular concentrations of taurine and glutamate. Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate in a PKC activity- dependent manner. Plays a role in the retention of SLC1A1/EAAC1 in the endoplasmic reticulum.
Cellular Location Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9ES40}; Multi-pass membrane protein. Cell membrane {ECO:0000250|UniProtKB:Q9ES40}; Multi-pass membrane protein. Cytoplasm {ECO:0000250|UniProtKB:Q9ES40}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q9ES40}. Note=Also exists as a soluble form in the cytoplasm. Associated with microtubules {ECO:0000250|UniProtKB:Q9ES40}
Research Areas
Citations (0)
citation

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Background

Expression of this gene is affected by vitamin A. The encoded protein of this gene may be associated with the cytoskeleton. A similar protein in rats may play a role in the regulation of cell differentiation. The rat protein binds and inhibits the cell membrane glutamate transporter EAAC1. The expression of the rat gene is upregulated by retinoic acid, which results in a specific reduction in EAAC1-mediated glutamate transport.

References

Genome-wide association study of alcohol dependence implicates a region on chromosome 11. Edenberg HJ, et al. Alcohol Clin Exp Res, 2010 May. PMID 20201924.
Modulation of the neural glutamate transporter EAAC1 by the addicsin-interacting protein ARL6IP1. Akiduki S, et al. J Biol Chem, 2008 Nov 14. PMID 18684713.
The endoplasmic reticulum exit of glutamate transporter is regulated by the inducible mammalian Yip6b/GTRAP3-18 protein. Ruggiero AM, et al. J Biol Chem, 2008 Mar 7. PMID 18167356.
Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.
Regulation of glutathione synthesis via interaction between glutamate transport-associated protein 3-18 (GTRAP3-18) and excitatory amino acid carrier-1 (EAAC1) at plasma membrane. Watabe M, et al. Mol Pharmacol, 2007 Nov. PMID 17646425.

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$ 216.00
Cat# AF1515a
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