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Goat Anti-HEC1 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-HEC1 Antibody AF1523a
    AF1523a staining (0.5 µg/ml) of Hela lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O14777
Other Accession NP_006092, 10403
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 73913 Da
Additional Information
Gene ID 10403
Other Names Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, HsHec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC, NDC80, HEC, HEC1, KNTC2
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-HEC1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDC80
Synonyms HEC, HEC1, KNTC2
Function Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Cellular Location Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore
Research Areas
Citations (0)

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HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.


Centrosome-related genes, genetic variation, and risk of breast cancer. Olson JE, et al. Breast Cancer Res Treat, 2010 May 28. PMID 20508983.
siRNA-mediated knockdown against CDCA1 and KNTC2, both frequently overexpressed in colorectal and gastric cancers, suppresses cell proliferation and induces apoptosis. Kaneko N, et al. Biochem Biophys Res Commun, 2009 Dec 25. PMID 19878654.
Hec1 contributes to mitotic centrosomal microtubule growth for proper spindle assembly through interaction with Hice1. Wu G, et al. Mol Biol Cell, 2009 Nov. PMID 19776357.
PinX1 is a novel microtubule-binding protein essential for accurate chromosome segregation. Yuan K, et al. J Biol Chem, 2009 Aug 21. PMID 19553660.
Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly. Chan YW, et al. J Cell Biol, 2009 Jun 1. PMID 19468067.

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$ 325.00
Cat# AF1523a
(40 western blots)
Availability: 7-10 days
Bulk Size
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