|Application ||WB, E|
|Other Accession||NP_006451, 10614, 192231 (mouse)|
|Calculated MW||40623 Da|
|Other Names||Protein HEXIM1, Cardiac lineage protein 1, Estrogen down-regulated gene 1 protein, Hexamethylene bis-acetamide-inducible protein 1, Menage a quatre protein 1, HEXIM1, CLP1, EDG1, HIS1, MAQ1|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-HEXIM1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CLP1, EDG1, HIS1, MAQ1|
|Function||Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.|
|Cellular Location||Nucleus Cytoplasm. Note=Binds alpha-importin and is mostly nuclear (PubMed:16362050)|
|Tissue Location||Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed Expressed in endocrine tissues.|
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Provided below are standard protocols that you may find useful for product applications.
Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns.
HEXIM1 modulates vascular endothelial growth factor expression and function in breast epithelial cells and mammary gland. Ogba N, et al. Oncogene, 2010 Jun 24. PMID 20453883.
A flexible bipartite coiled coil structure is required for the interaction of Hexim1 with the P-TEFB subunit cyclin T1. Sch枚nichen A, et al. Biochemistry, 2010 Apr 13. PMID 20210365.
T-loop phosphorylated Cdk9 localizes to nuclear speckle domains which may serve as sites of active P-TEFb function and exchange between the Brd4 and 7SK/HEXIM1 regulatory complexes. Dow EC, et al. J Cell Physiol, 2010 Jul. PMID 20201073.
Specificity of Hexim1 and Hexim2 complex formation with cyclin T1/T2, importin alpha and 7SK snRNA. Czudnochowski N, et al. J Mol Biol, 2010 Jan 8. PMID 19883659.
Ubiquitination of HEXIM1 by HDM2. Lau J, et al. Cell Cycle, 2009 Jul 15. PMID 19617712.
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