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Goat Anti-ILF2 / NF45 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-ILF2 / NF45 Antibody AF1564a
    AF1564a (0.03 µg/ml) staining of Human Tonsil lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • IHC - Goat Anti-ILF2 / NF45 Antibody AF1564a
    AF1564a (2.5 µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q12905
Other Accession NP_004506, 3608, 67781 (mouse), 310612 (rat)
Reactivity Human
Predicted Mouse, Rat, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5mg/ml
Isotype IgG
Calculated MW 43062 Da
Additional Information
Gene ID 3608
Other Names Interleukin enhancer-binding factor 2, Nuclear factor of activated T-cells 45 kDa, ILF2, NF45
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-ILF2 / NF45 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ILF2
Synonyms NF45
Function Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. Essential for the efficient reshuttling of ILF3 (isoform 1 and isoform 2) into the nucleus.
Cellular Location Nucleus, nucleolus. Cytoplasm. Nucleus. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs
Research Areas
Citations (0)

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Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.


New genetic associations detected in a host response study to hepatitis B vaccine. Davila S, et al. Genes Immun, 2010 Apr. PMID 20237496.
NF45 and NF90 regulate HS4-dependent interleukin-13 transcription in T cells. Kiesler P, et al. J Biol Chem, 2010 Mar 12. PMID 20051514.
NF45 functions as an IRES trans-acting factor that is required for translation of cIAP1 during the unfolded protein response. Graber TE, et al. Cell Death Differ, 2010 Apr. PMID 19893574.
The NF90-NF45 complex functions as a negative regulator in the microRNA processing pathway. Sakamoto S, et al. Mol Cell Biol, 2009 Jul. PMID 19398578.
Nuclear factor 45 (NF45) is a regulatory subunit of complexes with NF90/110 involved in mitotic control. Guan D, et al. Mol Cell Biol, 2008 Jul. PMID 18458058.

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$ 325.00
Cat# AF1564a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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