|Application ||WB, IHC, E|
|Other Accession||NP_004960, 3416|
|Calculated MW||117968 Da|
|Other Names||Insulin-degrading enzyme, 188.8.131.52, Abeta-degrading protease, Insulin protease, Insulinase, Insulysin, IDE|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-Insulysin / Insulinase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.|
|Cellular Location||Cytoplasm. Cell membrane. Secreted. Note=Present at the cell surface of neuron cells The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.
Association between variants in IDE-KIF11-HHEX and plasma amyloid beta levels. Reitz C, et al. Neurobiol Aging, 2010 Aug 17. PMID 20724036.
Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.
Type 2 diabetes risk alleles near ADCY5, CDKAL1 and HHEX-IDE are associated with reduced birthweight. Andersson EA, et al. Diabetologia, 2010 Sep. PMID 20490451.
BMI at age 8 years is influenced by the type 2 diabetes susceptibility genes HHEX-IDE and CDKAL1. Winkler C, et al. Diabetes, 2010 Aug. PMID 20460429.
Combining genetic markers and clinical risk factors improves the risk assessment of impaired glucose metabolism. Ruchat SM, et al. Ann Med, 2010 Apr. PMID 20384434.
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