|Application ||WB, IHC, E|
|Other Accession||NP_002259, 3840|
|Predicted||Rat, Dog, Cow|
|Calculated MW||57887 Da|
|Other Names||Importin subunit alpha-3, Importin alpha Q1, Qip1, Karyopherin subunit alpha-4, KPNA4, QIP1|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-KPNA4 / IPOA3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Highly expressed in testis, ovary, small intestine, heart, skeletal muscle, lung and pancreas, but barely detectable in kidney, thymus, colon and peripheral blood leukocytes|
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Provided below are standard protocols that you may find useful for product applications.
The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.
Importin alpha3 interacts with HIV-1 integrase and contributes to HIV-1 nuclear import and replication. Ao Z, et al. J Virol, 2010 Sep. PMID 20554775.
Common importin alpha specificity for papillomavirus E2 proteins. Bian XL, et al. Virus Res, 2010 Jun. PMID 20193720.
Stress-mediated nuclear stabilization of p53 is regulated by ubiquitination and importin-alpha3 binding. Marchenko ND, et al. Cell Death Differ, 2010 Feb. PMID 19927155.
Prohibitin inhibits tumor necrosis factor alpha-induced nuclear factor-kappa B nuclear translocation via the novel mechanism of decreasing importin alpha3 expression. Theiss AL, et al. Mol Biol Cell, 2009 Oct. PMID 19710421.
Plasmodium circumsporozoite protein promotes the development of the liver stages of the parasite. Singh AP, et al. Cell, 2007 Nov 2. PMID 17981117.
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