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Goat Anti-MBD2 (isoform 1) Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-MBD2 (isoform 1) Antibody AF1655a
    AF1655a (0.3 µg/ml) staining of Jurkat cell lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • IHC - Goat Anti-MBD2 (isoform 1) Antibody AF1655a
    AF1655a (2.5 µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UBB5
Other Accession NP_003918, 8932, 17191 (mouse)
Reactivity Human
Predicted Mouse, Rat, Dog
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 43255 Da
Additional Information
Gene ID 8932
Other Names Methyl-CpG-binding domain protein 2, Demethylase, DMTase, Methyl-CpG-binding protein MBD2, MBD2
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-MBD2 (isoform 1) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MBD2
Function Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
Cellular Location Nucleus. Note=Nuclear, in discrete foci Detected at replication foci in late S phase
Tissue Location Highly expressed in brain, heart, kidney, stomach, testis and placenta.
Research Areas
Citations (0)

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DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.


A Large-scale genetic association study of esophageal adenocarcinoma risk. Liu CY, et al. Carcinogenesis, 2010 Jul. PMID 20453000.
Genetic susceptibility to distinct bladder cancer subphenotypes. Guey LT, et al. Eur Urol, 2010 Feb. PMID 19692168.
Genetic analysis of diabetic nephropathy on chromosome 18 in African Americans: linkage analysis and dense SNP mapping. McDonough CW, et al. Hum Genet, 2009 Aug 19. PMID 19690890.
PTEN identified as important risk factor of chronic obstructive pulmonary disease. Hosgood HD 3rd, et al. Respir Med, 2009 Dec. PMID 19625176.
Polymorphisms in innate immunity genes and lung cancer risk in Xuanwei, China. Shen M, et al. Environ Mol Mutagen, 2009 May. PMID 19170196.

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$ 325.00
Cat# AF1655a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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