|Application ||WB, IHC, E|
|Other Accession||NP_062552, 56257|
|Calculated MW||74355 Da|
|Other Names||7SK snRNA methylphosphate capping enzyme, MePCE, 2.1.1.-, Bicoid-interacting protein 3 homolog, Bin3 homolog, MEPCE, BCDIN3|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-MEPCE Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it.|
|Tissue Location||Expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. Weakly or not expressed in other tissues|
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Provided below are standard protocols that you may find useful for product applications.
A capping-independent function of MePCE in stabilizing 7SK snRNA and facilitating the assembly of 7SK snRNP. Xue Y, et al. Nucleic Acids Res, 2010 Jan. PMID 19906723.
Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.
LARP7 is a stable component of the 7SK snRNP while P-TEFb, HEXIM1 and hnRNP A1 are reversibly associated. Krueger BJ, et al. Nucleic Acids Res, 2008 Apr. PMID 18281698.
Human capping enzyme promotes formation of transcriptional R loops in vitro. Kaneko S, et al. Proc Natl Acad Sci U S A, 2007 Nov 6. PMID 17978174.
Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme. Jeronimo C, et al. Mol Cell, 2007 Jul 20. PMID 17643375.
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