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Goat Anti-MID2 / TRIM1 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-MID2 / TRIM1 Antibody AF1671a
    AF1671a (1 µg/ml) staining of mouse heart lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • IHC - Goat Anti-MID2 / TRIM1 Antibody AF1671a
    AF1671a (5 µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UJV3
Other Accession NP_438112, 11043, 23947 (mouse)
Reactivity Human, Mouse
Predicted Rat, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 83210 Da
Additional Information
Gene ID 11043
Other Names Probable E3 ubiquitin-protein ligase MID2, 6.3.2.-, Midin-2, Midline defect 2, Midline-2, RING finger protein 60, Tripartite motif-containing protein 1, MID2, FXY2, RNF60, TRIM1
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-MID2 / TRIM1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MID2
Synonyms FXY2, RNF60, TRIM1
Function May play a role in microtubule stabilization.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Note=Microtubule-associated
Tissue Location Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine
Research Areas
Citations (0)

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The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms.


A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Lim J, et al. Cell, 2006 May 19. PMID 16713569.
An Xq22.3 duplication detected by comparative genomic hybridization microarray (Array-CGH) defines a new locus (FGS5) for FG syndrome. Jehee FS, et al. Am J Med Genet A, 2005 Dec 15. PMID 16283679.
Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514.
The DNA sequence of the human X chromosome. Ross MT, et al. Nature, 2005 Mar 17. PMID 15772651.
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.

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$ 325.00
Cat# AF1671a
(40 western blots)
Availability: 7-10 days
Bulk Size
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