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Goat Anti-MYF5 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-MYF5 Antibody AF1703a
    AF1703a (0.5 µg/ml) staining of Muscle lysate (35 µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13349
Other Accession NP_005584, 4617
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 28296 Da
Additional Information
Gene ID 4617
Other Names Myogenic factor 5, Myf-5, Class C basic helix-loop-helix protein 2, bHLHc2, MYF5, BHLHC2
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-MYF5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MYF5
Synonyms BHLHC2
Function Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Cellular Location Nucleus.
Research Areas
Citations (0)

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DUX4c is up-regulated in FSHD. It induces the MYF5 protein and human myoblast proliferation. Ansseau E, et al. PLoS One, 2009 Oct 15. PMID 19829708.
High-density association study of 383 candidate genes for volumetric BMD at the femoral neck and lumbar spine among older men. Yerges LM, et al. J Bone Miner Res, 2009 Dec. PMID 19453261.
Altered binding of MYF-5 to FOXE1 promoter in non-syndromic and CHARGE-associated cleft palate. Venza M, et al. J Oral Pathol Med, 2009 Jan. PMID 19192046.
Efficacy of 3 days/wk resistance training on myofiber hypertrophy and myogenic mechanisms in young vs. older adults. Kosek DJ, et al. J Appl Physiol, 2006 Aug. PMID 16614355.
Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions. Suzuki Y, et al. Genome Res, 2004 Sep. PMID 15342556.

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$ 325.00
Cat# AF1703a
(40 western blots)
Availability: 7-10 days
Bulk Size
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