|Application ||WB, E|
|Other Accession||NP_004136, 4650|
|Calculated MW||243401 Da|
|Other Names||Unconventional myosin-IXb, Unconventional myosin-9b, MYO9B, MYR5|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-Myosin IXB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions (PubMed:9490638). Also acts as a GTPase activator for RHOA (PubMed:9490638, PubMed:26529257). Plays a role in the regulation of cell migration via its role as RHOA GTPase activator. This is regulated by its interaction with the SLIT2 receptor ROBO1; interaction with ROBO1 impairs interaction with RHOA and subsequent activation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed:26529257).|
|Cellular Location||Cytoplasm, cell cortex. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Note=In undifferentiated cells colocalizes with F-actin in the cell periphery while in differentiated cells its localization is cytoplasmic with the highest levels in the perinuclear region|
|Tissue Location||Detected in peripheral blood leukocytes (at protein level) (PubMed:9490638). Expressed predominantly in peripheral blood leukocytes and at lower levels, in thymus, spleen, testis, prostate, ovary, brain, small intestine and lung|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the myosin family of actin-based molecular motor heavy chain proteins. The protein has four IQ motifs located in the neck domain that bind calmodulin, which serves as a light chain. The protein complex has a single-headed structure and exhibits processive movement on actin filaments toward the minus-end. The protein also has rho-GTPase activity. Polymorphisms in this gene are associated with celiac disease and ulcerative colitis susceptibility. Multiple transcript variants encoding different isoforms have been found for this gene.
Association analysis of myosin IXB and type 1 diabetes. Persengiev S, et al. Hum Immunol, 2010 Jun. PMID 20303373.
Intestinal barrier gene variants may not explain the increased levels of antigliadin antibodies, suggesting other mechanisms than altered permeability. Wolters VM, et al. Hum Immunol, 2010 Apr. PMID 20096742.
Mutation of ARHGAP9 in patients with coronary spastic angina. Takefuji M, et al. J Hum Genet, 2010 Jan. PMID 19911011.
Association between genetic variants in myosin IXB and Crohn's disease. Cooney R, et al. Inflamm Bowel Dis, 2009 Jul. PMID 19235913.
MYO9B polymorphisms in multiple sclerosis. Kemppinen A, et al. Eur J Hum Genet, 2009 Jun. PMID 19142207.
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