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Goat Anti-NCF1 / p47phox Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-NCF1 / p47phox Antibody AF1709a
    AF1709a (0.2 µg/ml) staining of U937 lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14598
Other Accession NP_000256, 653361, 17969 (mouse), 114553 (rat)
Reactivity Human
Predicted Mouse, Rat, Rabbit, Pig, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 44652 Da
Additional Information
Gene ID 653361
Other Names Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox, NCF1, NOXO2, SH3PXD1A
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-NCF1 / p47phox Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCF1
Synonyms NOXO2, SH3PXD1A
Function NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Cellular Location Cytoplasm, cytosol. Membrane; Peripheral membrane protein; Cytoplasmic side
Tissue Location Detected in peripheral blood monocytes and neutrophils (at protein level).
Research Areas
Citations (0)

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The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.


Hematologically important mutations: the autosomal recessive forms of chronic granulomatous disease (second update). Roos D, et al. Blood Cells Mol Dis, 2010 Apr 15. PMID 20167518.
Integrative predictive model of coronary artery calcification in atherosclerosis. McGeachie M, et al. Circulation, 2009 Dec 15. PMID 19948975.
NOX activity is increased in mild cognitive impairment. Bruce-Keller AJ, et al. Antioxid Redox Signal, 2010 Jun 15. PMID 19929442.
Dynamin 2 and c-Abl are novel regulators of hyperoxia-mediated NADPH oxidase activation and reactive oxygen species production in caveolin-enriched microdomains of the endothelium. Singleton PA, et al. J Biol Chem, 2009 Dec 11. PMID 19833721.
Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening. Voss M, et al. BMC Immunol, 2009 Oct 6. PMID 19807924.

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$ 325.00
Cat# AF1709a
(40 western blots)
Availability: 7-10 days
Bulk Size
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