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Goat Anti-NCF4 / P40PHOX Antibody

Peptide-affinity purified goat antibody

  • IHC - Goat Anti-NCF4 / P40PHOX Antibody AF1710a
    AF1710a (2.5 µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • WB - Goat Anti-NCF4 / P40PHOX Antibody AF1710a
    AF1710a (0.5 µg/ml) staining of Daudi cell lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • WB - Goat Anti-NCF4 / P40PHOX Antibody AF1710a
    HEK293 overexpressing P40PHOX (RC201191) and probed with AF1710a (mock transfection in first lane), tested by Origene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15080
Other Accession NP_038202, 4689
Reactivity Human
Predicted Dog
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 39032 Da
Additional Information
Gene ID 4689
Other Names Neutrophil cytosol factor 4, NCF-4, Neutrophil NADPH oxidase factor 4, SH3 and PX domain-containing protein 4, p40-phox, p40phox, NCF4, SH3PXD4
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-NCF4 / P40PHOX Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCF4
Synonyms SH3PXD4
Function Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.
Cellular Location Cytoplasm, cytosol. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Membrane; Peripheral membrane protein
Tissue Location Expression is restricted to hematopoietic cells
Research Areas
Citations (0)

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The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.


Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.
Polymorphisms in innate immunity genes and risk of childhood leukemia. Han S, et al. Hum Immunol, 2010 Jul. PMID 20438785.
Risk of meningioma and common variation in genes related to innate immunity. Rajaraman P, et al. Cancer Epidemiol Biomarkers Prev, 2010 May. PMID 20406964.
Hematologically important mutations: the autosomal recessive forms of chronic granulomatous disease (second update). Roos D, et al. Blood Cells Mol Dis, 2010 Apr 15. PMID 20167518.
Polymorphisms in innate immunity genes and patients response to dendritic cell-based HIV immuno-treatment. Segat L, et al. Vaccine, 2010 Mar 2. PMID 20056178.

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$ 325.00
Cat# AF1710a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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