|Application ||IHC, E|
|Other Accession||NP_001040625, 10276, 56349 (mouse), 307098 (rat)|
|Predicted||Mouse, Rat, Dog|
|Calculated MW||67740 Da|
|Other Names||Neuroepithelial cell-transforming gene 1 protein, Proto-oncogene p65 Net1, Rho guanine nucleotide exchange factor 8, NET1, ARHGEF8|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-NET1 / ARHGEF8 (Internal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Widely expressed.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.
Interaction of the RhoA exchange factor Net1 with discs large homolog 1 protects it from proteasome-mediated degradation and potentiates Net1 activity. Carr HS, et al. J Biol Chem, 2009 Sep 4. PMID 19586902.
Coexpression of alpha6beta4 integrin and guanine nucleotide exchange factor Net1 identifies node-positive breast cancer patients at high risk for distant metastasis. Gilcrease MZ, et al. Cancer Epidemiol Biomarkers Prev, 2009 Jan. PMID 19124484.
NET1-mediated RhoA activation facilitates lysophosphatidic acid-induced cell migration and invasion in gastric cancer. Murray D, et al. Br J Cancer, 2008 Oct 21. PMID 18827818.
A bacterial cytotoxin identifies the RhoA exchange factor Net1 as a key effector in the response to DNA damage. Guerra L, et al. PLoS One, 2008 May 28. PMID 18509476.
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