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Goat Anti-NFIL3 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-NFIL3 Antibody AF1729a
    AF1729a staining (0.5 µg/ml) of HepG2 lysate (RIPA buffer, 20 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16649
Other Accession NP_005375, 4783, 18030 (mouse), 114519 (rat)
Reactivity Human
Predicted Mouse, Rat
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 51472 Da
Additional Information
Gene ID 4783
Other Names Nuclear factor interleukin-3-regulated protein, E4 promoter-binding protein 4, Interleukin-3 promoter transcriptional activator, Interleukin-3-binding protein 1, Transcriptional activator NF-IL3A, NFIL3, E4BP4, IL3BP1
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-NFIL3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NFIL3
Synonyms E4BP4, IL3BP1
Function Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity). Represses transcriptional activity of PER1 (By similarity). Represses transcriptional activity of PER2 via the B- site on the promoter (By similarity). Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity). Protects pro-B cells from programmed cell death (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00978}
Tissue Location Expressed in bladder stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and muscle
Research Areas
Citations (0)

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Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).


Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
Systematic analysis of circadian genes in a population-based sample reveals association of TIMELESS with depression and sleep disturbance. Utge SJ, et al. PLoS One, 2010 Feb 18. PMID 20174623.
Association study of 21 circadian genes with bipolar I disorder, schizoaffective disorder, and schizophrenia. Mansour HA, et al. Bipolar Disord, 2009 Nov. PMID 19839995.
PER2 variantion is associated with depression vulnerability. Lavebratt C, et al. Am J Med Genet B Neuropsychiatr Genet, 2010 Mar 5. PMID 19693801.
Identification of adjacent binding sites for the YY1 and E4BP4 transcription factors in the ovine PrP (Prion) gene promoter. Burgess ST, et al. J Biol Chem, 2009 Mar 13. PMID 19129193.

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$ 325.00
Cat# AF1729a
(40 western blots)
Availability: 7-10 days
Bulk Size
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