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Goat Anti-NHERF2 (isoform a) Antibody

Peptide-affinity purified goat antibody

  • IHC - Goat Anti-NHERF2 (isoform a) Antibody AF1731a
    AF1731a (3 µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
  • WB - Goat Anti-NHERF2 (isoform a) Antibody AF1731a
    AF1731a (0.3 µg/ml) staining of human kidney lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15599
Other Accession NP_001123484, 9351
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 37414 Da
Additional Information
Gene ID 9351
Other Names Na(+)/H(+) exchange regulatory cofactor NHE-RF2, NHERF-2, NHE3 kinase A regulatory protein E3KARP, SRY-interacting protein 1, SIP-1, Sodium-hydrogen exchanger regulatory factor 2, Solute carrier family 9 isoform A3 regulatory factor 2, Tyrosine kinase activator protein 1, TKA-1, SLC9A3R2, NHERF2
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-NHERF2 (isoform a) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SLC9A3R2
Synonyms NHERF2
Function Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus.
Cellular Location Endomembrane system; Peripheral membrane protein. Nucleus. Apical cell membrane Note=Localizes with EZR and PODXL at the apical cell membrane of glomerular epithelium cells and the sides of the food processes (By similarity). Nuclear, in a punctate pattern.
Tissue Location Widely expressed.
Research Areas
Citations (0)

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NHE3 (SLC9A3; MIM 182307) is a sodium/hydrogen exchanger in the brush border membrane of the proximal tubule, small intestine, and colon that plays a major role in transepithelial sodium absorption. SLC9A3R2, as well as SLC9A3R1 (MIM 604990) and protein kinase A phosphorylation, may play a role in NHE3 regulation.


Na/H exchanger regulatory factors control parathyroid hormone receptor signaling by facilitating differential activation of G(alpha) protein subunits. Wang B, et al. J Biol Chem, 2010 Aug 27. PMID 20562104.
Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.
MCC, a new interacting protein for Scrib, is required for cell migration in epithelial cells. Arnaud C, et al. FEBS Lett, 2009 Jul 21. PMID 19555689.
Lysophosphatidic acid 2 receptor-mediated supramolecular complex formation regulates its antiapoptotic effect. E S, et al. J Biol Chem, 2009 May 22. PMID 19293149.
Regulation of protein expression and function of octn2 in forskolin-induced syncytialization in BeWo Cells. Huang FD, et al. Placenta, 2009 Feb. PMID 19091402.

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$ 325.00
Cat# AF1731a
(40 western blots)
Availability: 7-10 days
Bulk Size
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