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Goat Anti-NIPP1 / PPP1R8 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-NIPP1 / PPP1R8 Antibody AF1732a
    AF1732a staining (2 µg/ml) of H460 lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • IHC - Goat Anti-NIPP1 / PPP1R8 Antibody AF1732a
    AF1732a (3.8 µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q12972
Other Accession NP_612568, 5511, 100336 (mouse)
Reactivity Human
Predicted Mouse, Rat, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 38479 Da
Additional Information
Gene ID 5511
Other Names Nuclear inhibitor of protein phosphatase 1, NIPP-1, Protein phosphatase 1 regulatory inhibitor subunit 8, Activator of RNA decay, 3.1.4.-, ARD-1, PPP1R8, ARD1, NIPP1
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-NIPP1 / PPP1R8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPP1R8
Synonyms ARD1, NIPP1
Function Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.
Cellular Location Nucleus. Nucleus speckle. Note=Primarily, but not exclusively, nuclear
Tissue Location Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells
Research Areas
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$ 325.00
Cat# AF1732a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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