|Application ||IHC, E|
|Other Accession||NP_751907, 124056|
|Calculated MW||41253 Da|
|Other Names||NADPH oxidase organizer 1, NADPH oxidase regulatory protein, Nox organizer 1, Nox-organizing protein 1, SH3 and PX domain-containing protein 5, NOXO1, P41NOX, SH3PXD5|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-NOXO1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity.|
|Cellular Location||Isoform 3: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Isoform 3 associates with the plasma membrane in a lipid-dependent manner (PubMed:12716910)|
|Tissue Location||Expressed in testis, small and large intestines, liver, kidney and pancreas. Isoform 3 is mainly expressed in colon. Isoform 1 is preferentially expressed in testis.|
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Provided below are standard protocols that you may find useful for product applications.
NADPH oxidases (NOXs) catalyze the transfer of electrons from NADPH to molecular oxygen to generate reactive oxygen species (ROS). NOX organizers, such as NOXO1, target NOX activators (see NOXA1; MIM 611255) to NOX and also target NOX to different subcellular compartments (Opitz et al., 2007 [PubMed 17189823]).
Regulation of NOXO1 activity through reversible interactions with p22 and NOXA1. Dutta S, et al. PLoS One, 2010 May 4. PMID 20454568.
The 'A's and 'O's of NADPH oxidase regulation: a commentary on Subcellular localization and function of alternatively spliced Noxo1 isoforms. Opitz N, et al. Free Radic Biol Med, 2007 Jan 15. PMID 17189823.
Interaction between the SH3 domains and C-terminal proline-rich region in NADPH oxidase organizer 1 (Noxo1). Yamamoto A, et al. Biochem Biophys Res Commun, 2007 Jan 12. PMID 17126813.
Expression and function of Noxo1gamma, an alternative splicing form of the NADPH oxidase organizer 1. Takeya R, et al. FEBS J, 2006 Aug. PMID 16911517.
Nox1-dependent reactive oxygen generation is regulated by Rac1. Cheng G, et al. J Biol Chem, 2006 Jun 30. PMID 16636067.
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