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Goat Anti-ORAI1 / CRACM1 Antibody
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q96D31 |
| Other Accession | NP_116179, 84876, 109305 (mouse) |
| Reactivity | Human |
| Predicted | Pig, Dog |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 100ug/200ul |
| Isotype | IgG |
| Calculated MW | 32668 Da |
| Gene ID | 84876 |
|---|---|
| Other Names | Calcium release-activated calcium channel protein 1, Protein orai-1, Transmembrane protein 142A, ORAI1, CRACM1, TMEM142A |
| Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Goat Anti-ORAI1 / CRACM1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ORAI1 |
|---|---|
| Synonyms | CRACM1, TMEM142A |
| Function | Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1 (PubMed:16582901, PubMed:16645049, PubMed:16733527, PubMed:16766533, PubMed:16807233, PubMed:19249086, PubMed:23307288, PubMed:24351972, PubMed:24591628, PubMed:28219928, PubMed:20354224, PubMed:26956484). CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT (PubMed:16582901). Plays a prominent role in Ca(2+) influx at the basolateral membrane of mammary epithelial cells independently of the Ca(2+) content of endoplasmic reticulum or Golgi stores. May mediate transepithelial transport of large quantities of Ca(2+) for milk secretion. |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Basolateral cell membrane {ECO:0000250|UniProtKB:Q8BWG9}; Multi-pass membrane protein. Note=Isoform beta is more mobile in the plasma membrane (PubMed:23307288). Colocalizes with STIM1 at the cell membrane (PubMed:27185316). |
| Tissue Location | Expressed in naive CD4 and CD8 T cells (at protein level) (PubMed:26956484). Expressed at similar levels in naive and effector T helper cells (PubMed:20354224) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
CRACM1 is a plasma membrane protein essential for store-operated calcium entry (Vig et al., 2006 [PubMed 16645049]).
References
Protein kinase C-induced phosphorylation of Orai1 regulates the intracellular Ca2+ level via the store-operated Ca2+ channel. Kawasaki T, et al. J Biol Chem, 2010 Aug 13. PMID 20534587.
A novel native store-operated calcium channel encoded by Orai3: selective requirement of Orai3 versus Orai1 in estrogen receptor-positive versus estrogen receptor-negative breast cancer cells. Motiani RK, et al. J Biol Chem, 2010 Jun 18. PMID 20395295.
Differential redox regulation of ORAI ion channels: a mechanism to tune cellular calcium signaling. Bogeski I, et al. Sci Signal, 2010 Mar 30. PMID 20354224.
Pore architecture of the ORAI1 store-operated calcium channel. Zhou Y, et al. Proc Natl Acad Sci U S A, 2010 Mar 16. PMID 20194792.
Expression and association of TRPC subtypes with Orai1 and STIM1 in human parathyroid. Lu M, et al. J Mol Endocrinol, 2010 May. PMID 20194530.
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