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Goat Anti-PACSIN3 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-PACSIN3 Antibody AF1775a
    AF1775a staining (1 µg/ml) of H460 lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UKS6
Other Accession NP_057307, 29763, 80708 (mouse), 311187 (rat)
Reactivity Human
Predicted Mouse, Rat
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 48487 Da
Additional Information
Gene ID 29763
Other Names Protein kinase C and casein kinase substrate in neurons protein 3, SH3 domain-containing protein 6511, PACSIN3
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-PACSIN3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity (By similarity).
Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Detected at the inner aspect of the plasma membrane in myotubes
Tissue Location Widely expressed, with highest levels in heart and skeletal muscle, intermediate levels in placenta, liver and pancreas, and very low levels in brain, lung and kidney
Research Areas
Citations (0)

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This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation. Alternative splicing results in multiple transcript variants.


Identification of interaction partners for individual SH3 domains of Fas ligand associated members of the PCH protein family in T lymphocytes. Linkermann A, et al. Biochim Biophys Acta, 2009 Feb. PMID 19041431.
Exploration of human ORFeome: high-throughput preparation of ORF clones and efficient characterization of their protein products. Nagase T, et al. DNA Res, 2008 Jun 30. PMID 18316326.
Stimulus-specific modulation of the cation channel TRPV4 by PACSIN 3. D'hoedt D, et al. J Biol Chem, 2008 Mar 7. PMID 18174177.
Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Olsen JV, et al. Cell, 2006 Nov 3. PMID 17081983.

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$ 325.00
Cat# AF1775a
(40 western blots)
Availability: 7-10 days
Bulk Size
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