|Application ||WB, E|
|Other Accession||NP_036519, 23569|
|Calculated MW||74079 Da|
|Other Names||Protein-arginine deiminase type-4, 22.214.171.124, HL-60 PAD, Peptidylarginine deiminase IV, Protein-arginine deiminase type IV, PADI4, PADI5, PDI5|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-PAD4 / PADI4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||PAD4, PADI5, PDI5|
|Function||Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg- 54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.|
|Cellular Location||Cytoplasm. Nucleus. Cytoplasmic granule. Note=Cytoplasmic granules of eosinophils and neutrophils|
|Tissue Location||Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes|
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Provided below are standard protocols that you may find useful for product applications.
This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response.
The PADI4 gene does not contribute to genetic susceptibility to rheumatoid arthritis in Chinese Han population. Chen R, et al. Rheumatol Int, 2010 Jun 20. PMID 20563870.
Peptidyl arginine deiminase type IV (PADI4) haplotypes interact with shared epitope regardless of anti-cyclic citrullinated peptide antibody or erosive joint status in rheumatoid arthritis: a case control study. Bang SY, et al. Arthritis Res Ther, 2010. PMID 20537173.
Contribution of anti-CCP antibodies, proximal interphalangeal joint involvement, HLA-DRB1 shared epitope, and PADI4 as risk factors for the development of rheumatoid arthritis in palindromic rheumatism. Tamai M, et al. Scand J Rheumatol, 2010 Aug. PMID 20476860.
Cumulative association of 22 genetic variants with seropositive rheumatoid arthritis risk. Karlson EW, et al. Ann Rheum Dis, 2010 Jun. PMID 20233754.
Coordination of PAD4 and HDAC2 in the regulation of p53-target gene expression. Li P, et al. Oncogene, 2010 May 27. PMID 20190809.
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