|Application ||WB, E|
|Other Accession||NP_620177, 5066|
|Calculated MW||108332 Da|
|Other Names||Peptidyl-glycine alpha-amidating monooxygenase, PAM, Peptidylglycine alpha-hydroxylating monooxygenase, PHM, 18.104.22.168, Peptidyl-alpha-hydroxyglycine alpha-amidating lyase, 22.214.171.124, Peptidylamidoglycolate lyase, PAL, PAM|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-PAM / PAL Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.|
|Cellular Location||Isoform 1: Membrane; Single-pass type I membrane protein Isoform 3: Secreted. Note=Secreted from secretory granules|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene but some of their full length sequences are not yet known.
E3 ligases Arf-bp1 and Pam mediate lithium-stimulated degradation of the circadian heme receptor Rev-erb alpha. Yin L, et al. Proc Natl Acad Sci U S A, 2010 Jun 22. PMID 20534529.
Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
Poor replication of candidate genes for major depressive disorder using genome-wide association data. Bosker FJ, et al. Mol Psychiatry, 2010 Mar 30. PMID 20351714.
The peptidylglycine alpha-amidating monooxygenase (PAM): a novel prodrug strategy for amidoximes and N-hydroxyguanidines? Schade D, et al. ChemMedChem, 2009 Oct. PMID 19693765.
Common variants conferring risk of schizophrenia. Stefansson H, et al. Nature, 2009 Aug 6. PMID 19571808.
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