|Application ||WB, E|
|Other Accession||NP_002601, 5163|
|Predicted||Mouse, Pig, Dog, Cow|
|Calculated MW||49244 Da|
|Other Names||[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial, 188.8.131.52, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1, PDK1, PDHK1|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-PDK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.|
|Cellular Location||Mitochondrion matrix|
|Tissue Location||Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas|
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Provided below are standard protocols that you may find useful for product applications.
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.
Identification of type 2 diabetes-associated combination of SNPs using support vector machine. Ban HJ, et al. BMC Genet, 2010 Apr 23. PMID 20416077.
Pyruvate dehydrogenase kinase 1 controls mitochondrial metabolism and insulin secretion in INS-1 832/13 clonal beta-cells. Krus U, et al. Biochem J, 2010 Jul 1. PMID 20415663.
Discovery of PDK1 kinase inhibitors with a novel mechanism of action by ultrahigh throughput screening. Bobkova EV, et al. J Biol Chem, 2010 Jun 11. PMID 20385558.
3-phosphoinositide-dependent protein kinase-1 regulates proliferation and survival of cancer cells with an activated mitogen-activated protein kinase pathway. Lu Z, et al. Mol Cancer Res, 2010 Mar. PMID 20197379.
Reciprocal negative regulation of PDK1 and ASK1 signaling by direct interaction and phosphorylation. Seong HA, et al. J Biol Chem, 2010 Jan 22. PMID 19920149.
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