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Goat Anti-Pericentrin 1 / NUP85 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-Pericentrin 1 / NUP85 Antibody AF1811a
    AF1811a (1 µg/ml) staining of Hela lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BW27
Other Accession NP_079120, 79902, 18541 (mouse)
Reactivity Human
Predicted Mouse, Rat
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 75019 Da
Additional Information
Gene ID 79902
Other Names Nuclear pore complex protein Nup85, 85 kDa nucleoporin, FROUNT, Nucleoporin Nup75, Nucleoporin Nup85, Pericentrin-1, NUP85, NUP75, PCNT1
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-Pericentrin 1 / NUP85 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NUP85
Synonyms NUP75, PCNT1
Function Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2- activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl- inositol 3-kinase-Rac-lammellipodium protrusion cascade.
Cellular Location Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle Cytoplasm. Nucleus membrane. Note=During mitosis, localizes to the kinetochores and spindle poles. Upon CCl2 stimulation translocates from the cytoplasm to the membrane and colocalizes with CCR2 at the front of migrating cells
Research Areas
Citations (0)

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Bidirectional transport of macromolecules between the cytoplasm and nucleus occurs through nuclear pore complexes (NPCs) embedded in the nuclear envelope. NPCs are composed of subcomplexes, and NUP85 is part of one such subcomplex, Nup107-160.


FROUNT is a common regulator of CCR2 and CCR5 signaling to control directional migration. Toda E, et al. J Immunol, 2009 Nov 15. PMID 19841162.
Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.
The structure of the scaffold nucleoporin Nup120 reveals a new and unexpected domain architecture. Leksa NC, et al. Structure, 2009 Aug 12. PMID 19576787.
A novel activator of C-C chemokine, FROUNT, is expressed with C-C chemokine receptor 2 and its ligand in failing human heart. Satoh M, et al. J Card Fail, 2007 Mar. PMID 17395051.
The human Nup107-160 nuclear pore subcomplex contributes to proper kinetochore functions. Zuccolo M, et al. EMBO J, 2007 Apr 4. PMID 17363900.

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$ 325.00
Cat# AF1811a
(40 western blots)
Availability: 7-10 days
Bulk Size
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