|Application ||WB, IF, E|
|Other Accession||NP_001017408, 57120|
|Calculated MW||50520 Da|
|Other Names||Golgi-associated PDZ and coiled-coil motif-containing protein, CFTR-associated ligand, Fused in glioblastoma, PDZ protein interacting specifically with TC10, PIST, GOPC, CAL, FIG|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-PIST / FIG / GOPC Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.|
|Cellular Location||Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, dendrite. Note=Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides.|
|Tissue Location||Ubiquitously expressed.|
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Provided below are standard protocols that you may find useful for product applications.
PIST is a PDZ domain-containing Golgi protein. PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner.
Syntaxin 6 and CAL mediate the degradation of the cystic fibrosis transmembrane conductance regulator. Cheng J, et al. Mol Biol Cell, 2010 Apr 1. PMID 20130090.
The relative binding affinities of PDZ partners for CFTR: a biochemical basis for efficient endocytic recycling. Cushing PR, et al. Biochemistry, 2008 Sep 23. PMID 18754678.
Targeting CAL as a negative regulator of DeltaF508-CFTR cell-surface expression: an RNA interference and structure-based mutagenetic approach. Wolde M, et al. J Biol Chem, 2007 Mar 16. PMID 17158866.
Solution structure of GOPC PDZ domain and its interaction with the C-terminal motif of neuroligin. Li X, et al. Protein Sci, 2006 Sep. PMID 16882988.
Identification of a PDZ protein, PIST, as a binding partner for Rho effector Rhotekin: biochemical and cell-biological characterization of Rhotekin-PIST interaction. Ito H, et al. Biochem J, 2006 Aug 1. PMID 16646955.
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