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Goat Anti-Pleckstrin Antibody

Peptide-affinity purified goat antibody

     
  • WB - Goat Anti-Pleckstrin Antibody AF1840a
    AF1840a staining (0.01 µg/ml) of Human Parathyroid lysate (A) and negative control HepG2 (B) cell lysate (35 µg protein in RIPA buffer). . Detected by chemiluminescence.
    detail
  • IHC - Goat Anti-Pleckstrin Antibody AF1840a
    AF1840a (3.75 µg/ml) staining of paraffin embedded Human Vessel (platelets). Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
    detail
  • IHC - Goat Anti-Pleckstrin Antibody AF1840a
    AF1840a (3.75 µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
    detail
  • IF - Goat Anti-Pleckstrin Antibody AF1840a
    AF1840a Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane and nuclear staining. The nuc
    detail
  • FC - Goat Anti-Pleckstrin Antibody AF1840a
    AF1840a Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P08567
Other Accession NP_002655, 5341
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 40125 Da
Additional Information
Gene ID 5341
Other Names Pleckstrin, Platelet 47 kDa protein, p47, PLEK, P47
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-Pleckstrin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLEK
Synonyms P47
Function Major protein kinase C substrate of platelets.
Research Areas
Citations (0)
citation

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References

Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.
Multiple common variants for celiac disease influencing immune gene expression. Dubois PC, et al. Nat Genet, 2010 Apr. PMID 20190752.
Novel role of pleckstrin homology domain of the Bcr-Abl protein: analysis of protein-protein and protein-lipid interactions. Miroshnychenko D, et al. Exp Cell Res, 2010 Feb 15. PMID 19944685.
Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. Talmud PJ, et al. Am J Hum Genet, 2009 Nov. PMID 19913121.
Proteomic identification of pleckstrin-associated proteins in platelets: possible interactions with actin. Baig A, et al. Proteomics, 2009 Sep. PMID 19722192.

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$ 341.00
Cat# AF1840a
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