|Application ||WB, IHC, E|
|Other Accession||NP_055145, 23645|
|Calculated MW||73478 Da|
|Other Names||Protein phosphatase 1 regulatory subunit 15A, Growth arrest and DNA damage-inducible protein GADD34, Myeloid differentiation primary response protein MyD116 homolog, PPP1R15A, GADD34|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-PPP1R15A / GADD34 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.|
|Cellular Location||Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associates with membranes via an N-terminal amphipathic intramembrane region|
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Provided below are standard protocols that you may find useful for product applications.
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation.
EBNA3C interacts with Gadd34 and counteracts the unfolded protein response. Garrido JL, et al. Virol J, 2009 Dec 29. PMID 20040105.
Disruption of the PP1/GADD34 complex induces calreticulin exposure. Kepp O, et al. Cell Cycle, 2009 Dec. PMID 19901557.
Inhibition of protein kinase R activation and upregulation of GADD34 expression play a synergistic role in facilitating coronavirus replication by maintaining de novo protein synthesis in virus-infected cells. Wang X, et al. J Virol, 2009 Dec. PMID 19776135.
An upstream open reading frame regulates translation of GADD34 during cellular stresses that induce eIF2alpha phosphorylation. Lee YY, et al. J Biol Chem, 2009 Mar 13. PMID 19131336.
Control of cellular GADD34 levels by the 26S proteasome. Brush MH, et al. Mol Cell Biol, 2008 Dec. PMID 18794359.
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