|Application ||WB, E|
|Other Accession||NP_004147, 5515, 5516, 19052 (mouse), 24672 (rat)|
|Predicted||Mouse, Rat, Rabbit, Pig, Dog, Cow|
|Calculated MW||35594 Da|
|Other Names||Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, 184.108.40.206, Replication protein C, RP-C, PPP2CA|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-PPP2CA / PPP2CB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.|
|Cellular Location||Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. Note=In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGO2 (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.
An approach based on a genome-wide association study reveals candidate loci for narcolepsy. Shimada M, et al. Hum Genet, 2010 Oct. PMID 20677014.
Neuroprotectin D1 induces dephosphorylation of Bcl-xL in a PP2A-dependent manner during oxidative stress and promotes retinal pigment epithelial cell survival. Antony R, et al. J Biol Chem, 2010 Jun 11. PMID 20363734.
HUNK suppresses metastasis of basal type breast cancers by disrupting the interaction between PP2A and cofilin-1. Quintela-Fandino M, et al. Proc Natl Acad Sci U S A, 2010 Feb 9. PMID 20133759.
cAMP-stimulated protein phosphatase 2A activity associated with muscle A kinase-anchoring protein (mAKAP) signaling complexes inhibits the phosphorylation and activity of the cAMP-specific phosphodiesterase PDE4D3. Dodge-Kafka KL, et al. J Biol Chem, 2010 Apr 9. PMID 20106966.
Endotoxin conditioning induces VCP/p97-mediated and inducible nitric-oxide synthase-dependent Tyr284 nitration in protein phosphatase 2A. Ohama T, et al. J Biol Chem, 2010 Mar 19. PMID 20100830.
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