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Goat Anti-Pyruvate Carboxylase Antibody
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P11498 |
| Other Accession | NP_071504, 5091, 18563 (mouse), 25104 (rat) |
| Reactivity | Mouse |
| Predicted | Human, Rat, Pig, Dog, Cow |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 100ug/200ul |
| Isotype | IgG |
| Calculated MW | 129634 Da |
| Gene ID | 5091 |
|---|---|
| Other Names | Pyruvate carboxylase, mitochondrial, 6.4.1.1, Pyruvic carboxylase, PCB, PC |
| Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Goat Anti-Pyruvate Carboxylase Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PC (HGNC:8636) |
|---|---|
| Function | Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. |
| Cellular Location | Mitochondrion matrix |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.
References
Structural insights on pathogenic effects of novel mutations causing pyruvate carboxylase deficiency. Monnot S, et al. Hum Mutat, 2009 May. PMID 19306334.
Decreased levels of metabolic enzymes in pancreatic islets of patients with type 2 diabetes. MacDonald MJ, et al. Diabetologia, 2009 Jun. PMID 19296078.
Thrombophilia and unexplained pregnancy loss in Indian patients. Vora S, et al. Natl Med J India, 2008 May-Jun. PMID 19004141.
The molecular basis of pyruvate carboxylase deficiency: mosaicism correlates with prolonged survival. Wang D, et al. Mol Genet Metab, 2008 Sep-Oct. PMID 18676167.
Crystal structures of human and Staphylococcus aureus pyruvate carboxylase and molecular insights into the carboxyltransfer reaction. Xiang S, et al. Nat Struct Mol Biol, 2008 Mar. PMID 18297087.
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