|Application ||WB, E|
|Other Accession||NP_006316, 5901, 19384 (mouse), 84509 (rat)|
|Predicted||Rat, Dog, Cow|
|Calculated MW||24423 Da|
|Other Names||GTP-binding nuclear protein Ran, Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein, RAN, ARA24|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-RAN Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2.|
|Cellular Location||Nucleus. Cytoplasm. Melanosome. Note=Predominantly nuclear during interphase (By similarity) Becomes dispersed throughout the cytoplasm during mitosis Identified by mass spectrometry in melanosome fractions from stage I to stage IV.|
|Tissue Location||Expressed in a variety of tissues.|
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Provided below are standard protocols that you may find useful for product applications.
RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.
Association of a common AGO1 variant with lung cancer risk: A two-stage case-control study. Kim JS, et al. Mol Carcinog, 2010 Oct. PMID 20721975.
Role of primary miRNA polymorphic variants in metastatic colon cancer patients treated with 5-fluorouracil and irinotecan. Boni V, et al. Pharmacogenomics J, 2010 Jun 29. PMID 20585341.
The interaction of Epac1 and Ran promotes Rap1 activation at the nuclear envelope. Liu C, et al. Mol Cell Biol, 2010 Aug. PMID 20547757.
The Nup107-160 complex and gamma-TuRC regulate microtubule polymerization at kinetochores. Mishra RK, et al. Nat Cell Biol, 2010 Feb. PMID 20081840.
Activation of the Ran GTPase is subject to growth factor regulation and can give rise to cellular transformation. Ly TK, et al. J Biol Chem, 2010 Feb 19. PMID 20028979.
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