|Application ||WB, IHC, E|
|Other Accession||NP_002873, 5902|
|Calculated MW||23310 Da|
|Other Names||Ran-specific GTPase-activating protein, Ran-binding protein 1, RanBP1, RANBP1|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-RANBP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane.|
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Provided below are standard protocols that you may find useful for product applications.
Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.
Centrosome-related genes, genetic variation, and risk of breast cancer. Olson JE, et al. Breast Cancer Res Treat, 2010 May 28. PMID 20508983.
Importin-beta is a GDP-to-GTP exchange factor of Ran: implications for the mechanism of nuclear import. Lonhienne TG, et al. J Biol Chem, 2009 Aug 21. PMID 19549784.
RanBP1 downregulation sensitizes cancer cells to taxol in a caspase-3-dependent manner. Rensen WM, et al. Oncogene, 2009 Apr 16. PMID 19270727.
RANBP1 localizes a subset of mitotic regulatory factors on spindle microtubules and regulates chromosome segregation in human cells. Tedeschi A, et al. J Cell Sci, 2007 Nov 1. PMID 17940066.
Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.
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