|Application ||WB, IHC, E|
|Other Accession||NP_002874, 5905, 19387 (mouse)|
|Predicted||Rat, Dog, Cow|
|Calculated MW||63542 Da|
|Other Names||Ran GTPase-activating protein 1, RanGAP1, RANGAP1, KIAA1835, SD|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-RANGAP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.|
|Cellular Location||Cytoplasm. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Note=Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase Mitotic location also requires sumoylation|
|Tissue Location||Highly expressed in brain, thymus and testis.|
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Provided below are standard protocols that you may find useful for product applications.
RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle.
Structure of the Siz/PIAS SUMO E3 ligase Siz1 and determinants required for SUMO modification of PCNA. Yunus AA, et al. Mol Cell, 2009 Sep 11. PMID 19748360.
Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.
Mel-18 interacts with RanGAP1 and inhibits its sumoylation. Zhang J, et al. Biochem Biophys Res Commun, 2008 Oct 17. PMID 18706886.
Ubc9 sumoylation regulates SUMO target discrimination. Knipscheer P, et al. Mol Cell, 2008 Aug 8. PMID 18691969.
Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme. Jeronimo C, et al. Mol Cell, 2007 Jul 20. PMID 17643375.
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