|Application ||WB, IHC, E|
|Other Accession||NP_937883, 6249, 56430 (mouse), 65201 (rat)|
|Predicted||Mouse, Rat, Pig, Dog|
|Calculated MW||162246 Da|
|Other Names||CAP-Gly domain-containing linker protein 1, Cytoplasmic linker protein 1, Cytoplasmic linker protein 170 alpha-2, CLIP-170, Reed-Sternberg intermediate filament-associated protein, Restin, CLIP1, CYLN1, RSN|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-Restin / CLIP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking.|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle. Note=Associated with the cytoskeleton. Detected at the plus ends of microtubules in the cytosol, and close to plasma membrane ruffles. Associates with the membranes of intermediate macropinocytic vesicles|
|Tissue Location||Detected in dendritic cells (at protein level). Highly expressed in the Reed-Sternberg cells of Hodgkin disease.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Cdc2-mediated phosphorylation of CLIP-170 is essential for its inhibition of centrosome reduplication. Yang X, et al. J Biol Chem, 2009 Oct 16. PMID 19687009.
Microtubule plus-end tracking by CLIP-170 requires EB1. Dixit R, et al. Proc Natl Acad Sci U S A, 2009 Jan 13. PMID 19126680.
Dynein, Lis1 and CLIP-170 counteract Eg5-dependent centrosome separation during bipolar spindle assembly. Tanenbaum ME, et al. EMBO J, 2008 Dec 17. PMID 19020519.
The membrane-tubulating potential of amphiphysin 2/BIN1 is dependent on the microtubule-binding cytoplasmic linker protein 170 (CLIP-170). Meunier B, et al. Eur J Cell Biol, 2009 Feb. PMID 19004523.
Lamellar bodies of human epidermis: proteomics characterization by high throughput mass spectrometry and possible involvement of CLIP-170 in their trafficking/secretion. Raymond AA, et al. Mol Cell Proteomics, 2008 Nov. PMID 18622020.
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