|Application ||WB, E|
|Other Accession||NP_081075, 29102, 14000 (mouse), 310159 (rat)|
|Predicted||Mouse, Rat, Dog|
|Calculated MW||159316 Da|
|Other Names||Ribonuclease 3, 126.96.36.199, Protein Drosha, Ribonuclease III, RNase III, p241, DROSHA, RN3, RNASE3L, RNASEN|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-RNASEN / Drosha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||RN3, RNASE3L, RNASEN|
|Function||Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA- ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double- strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.|
|Cellular Location||Nucleus. Nucleus, nucleolus. Note=A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Canonical and alternate functions of the microRNA biogenesis machinery. Chong MM, et al. Genes Dev, 2010 Sep 1. PMID 20713509.
Tooth morphogenesis and ameloblast differentiation are regulated by micro-RNAs. Michon F, et al. Dev Biol, 2010 Apr 15. PMID 20102707.
Alternative processing of primary microRNA transcripts by Drosha generates 5' end variation of mature microRNA. Wu H, et al. PLoS One, 2009 Oct 27. PMID 19859542.
Genomic analysis suggests that mRNA destabilization by the microprocessor is specialized for the auto-regulation of Dgcr8. Shenoy A, et al. PLoS One, 2009 Sep 11. PMID 19759829.
Ars2 links the nuclear cap-binding complex to RNA interference and cell proliferation. Gruber JJ, et al. Cell, 2009 Jul 23. PMID 19632182.
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