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Goat Anti-RPE65 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-RPE65 Antibody AF1943a
    AF1943a (0.5 µg/ml) staining of Rat Retina lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16518
Other Accession NP_000320, 6121
Reactivity Rat
Predicted Human, Dog
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 60948 Da
Additional Information
Gene ID 6121
Other Names Retinoid isomerohydrolase,, All-trans-retinyl-palmitate hydrolase, Retinal pigment epithelium-specific 65 kDa protein, Retinol isomerase, RPE65
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-RPE65 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPE65
Function Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis- retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.
Cellular Location Cytoplasm. Cell membrane; Lipid-anchor. Microsome membrane. Note=Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated (By similarity). Undergoes light-dependent intracellular transport to become more concentrated in the central region of the retina pigment epithelium cells. {ECO:0000250, ECO:0000269|PubMed:21493626}
Tissue Location Retinal pigment epithelium specific.
Research Areas
Citations (0)

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This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa.


Development of a Diagnostic Genetic Test for Simplex and Autosomal Recessive Retinitis Pigmentosa. Clark GR, et al. Ophthalmology, 2010 Jun 28. PMID 20591486.
FATP1 inhibits 11-cis retinol formation via interaction with the visual cycle retinoid isomerase RPE65 and lecithin:retinol acyltransferase. Guignard TJ, et al. J Biol Chem, 2010 Jun 11. PMID 20356843.
Negative charge of the glutamic acid 417 residue is crucial for isomerohydrolase activity of RPE65. Nikolaeva O, et al. Biochem Biophys Res Commun, 2010 Jan 22. PMID 20043869.
Differential macular morphology in patients with RPE65-, CEP290-, GUCY2D-, and AIPL1-related Leber congenital amaurosis. Pasadhika S, et al. Invest Ophthalmol Vis Sci, 2010 May. PMID 19959640.
RPE65, visual cycle retinol isomerase, is not inherently 11-cis-specific: support for a carbocation mechanism of retinol isomerization. Redmond TM, et al. J Biol Chem, 2010 Jan 15. PMID 19920137.

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$ 325.00
Cat# AF1943a
(40 western blots)
Availability: 7-10 days
Bulk Size
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