|Application ||WB, E|
|Other Accession||NP_005137, 9092, 20227 (mouse), 29678 (rat)|
|Predicted||Mouse, Rat, Cow|
|Calculated MW||90255 Da|
|Other Names||U4/U6.U5 tri-snRNP-associated protein 1, SNU66 homolog, hSnu66, Squamous cell carcinoma antigen recognized by T-cells 1, SART-1, hSART-1, U4/U6.U5 tri-snRNP-associated 110 kDa protein, Hom s 1, SART1|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-SART1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA.|
|Cellular Location||Nucleus. Note=Found in the nucleus of mitogen- activated peripheral blood mononuclear cells (PBMCs), tumor cells, or normal cell lines, but not in normal tissues except testis and fetal liver or in unstimulated PBMCs, suggesting preferential expression in proliferating cells|
|Tissue Location||Ubiquitously expressed.|
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This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway.
Association of mitotic regulation pathway polymorphisms with pancreatic cancer risk and outcome. Couch FJ, et al. Cancer Epidemiol Biomarkers Prev, 2010 Jan. PMID 20056645.
Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.
Hypoxia-associated factor, a novel E3-ubiquitin ligase, binds and ubiquitinates hypoxia-inducible factor 1alpha, leading to its oxygen-independent degradation. Koh MY, et al. Mol Cell Biol, 2008 Dec. PMID 18838541.
Influence of multiple genetic polymorphisms on genitourinary morbidity after carbon ion radiotherapy for prostate cancer. Suga T, et al. Int J Radiat Oncol Biol Phys, 2008 Nov 1. PMID 18374504.
Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme. Jeronimo C, et al. Mol Cell, 2007 Jul 20. PMID 17643375.
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