|Application ||WB, E|
|Other Accession||NP_005841, 10262, 107701 (mouse)|
|Predicted||Mouse, Rat, Pig, Dog, Cow|
|Calculated MW||44386 Da|
|Other Names||Splicing factor 3B subunit 4, Pre-mRNA-splicing factor SF3b 49 kDa subunit, SF3b50, Spliceosome-associated protein 49, SAP 49, SF3B4, SAP49|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-SF3B4 / SAP49 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12- dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.
Many sequence variants affecting diversity of adult human height. Gudbjartsson DF, et al. Nat Genet, 2008 May. PMID 18391951.
Splicing factor 3b subunit 4 binds BMPR-IA and inhibits osteochondral cell differentiation. Watanabe H, et al. J Biol Chem, 2007 Jul 13. PMID 17513295.
Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening. Wu C, et al. Proteomics, 2007 Jun. PMID 17474147.
Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.
Human immunodeficiency virus type 1 Vpr induces G2 checkpoint activation by interacting with the splicing factor SAP145. Terada Y, et al. Mol Cell Biol, 2006 Nov. PMID 16923959.
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