|Application ||WB, E|
|Other Accession||NP_001129505, 22827, 67959 (mouse)|
|Predicted||Mouse, Rat, Dog, Cow|
|Calculated MW||59875 Da|
|Other Names||Poly(U)-binding-splicing factor PUF60, 60 kDa poly(U)-binding-splicing factor, FUSE-binding protein-interacting repressor, FBP-interacting repressor, Ro-binding protein 1, RoBP1, Siah-binding protein 1, Siah-BP1, PUF60, FIR, ROBPI, SIAHBP1|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-SIAHBP1 / FIR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||FIR, ROBPI, SIAHBP1|
|Function||DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.|
|Cellular Location||Nucleus. Note=Colocalizes partially with TROVE2|
|Tissue Location||Isoform 2 is expressed in colonic epithelium and colorectal epithelium cancer (at protein level). Isoform 6 is expressed in colorectal epithelial cancer but below detection level in colonic epithelium. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.
Quantitative characterization of the interactions among c-myc transcriptional regulators FUSE, FBP, and FIR. Hsiao HH, et al. Biochemistry, 2010 Jun 8. PMID 20420426.
Dimerization and protein binding specificity of the U2AF homology motif of the splicing factor Puf60. Corsini L, et al. J Biol Chem, 2009 Jan 2. PMID 18974054.
A protein-protein interaction network of transcription factors acting during liver cell proliferation. Gao J, et al. Genomics, 2008 Apr. PMID 18255255.
Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme. Jeronimo C, et al. Mol Cell, 2007 Jul 20. PMID 17643375.
Control of pre-mRNA splicing by the general splicing factors PUF60 and U2AF(65). Hastings ML, et al. PLoS One, 2007 Jun 20. PMID 17579712.
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