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Goat Anti-SIGLEC8 Antibody
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9NYZ4 |
| Other Accession | NP_055257, 27181 |
| Reactivity | Human |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 100ug/200ul |
| Isotype | IgG |
| Calculated MW | 54042 Da |
| Gene ID | 27181 |
|---|---|
| Other Names | Sialic acid-binding Ig-like lectin 8, Siglec-8, Sialoadhesin family member 2, SAF-2, SIGLEC8, SAF2 |
| Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Goat Anti-SIGLEC8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SIGLEC8 |
|---|---|
| Synonyms | SAF2 |
| Function | Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells (PubMed:10856141, PubMed:10625619). Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface (PubMed:10625619). Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6- sulfated sialyl-Lewis X glycan epitopes (PubMed:27357658). |
| Cellular Location | Membrane; Single-pass type I membrane protein. |
| Tissue Location | Expressed specifically on red blood cells namely basophil, mast cells and eosinophils. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).
References
New genetic associations detected in a host response study to hepatitis B vaccine. Davila S, et al. Genes Immun, 2010 Apr. PMID 20237496.
Polymorphisms in the sialic acid-binding immunoglobulin-like lectin-8 (Siglec-8) gene are associated with susceptibility to asthma. Gao PS, et al. Eur J Hum Genet, 2010 Jun. PMID 20087405.
Eosinophil-selective binding and proapoptotic effect in vitro of a synthetic Siglec-8 ligand, polymeric 6'-sulfated sialyl Lewis x. Hudson SA, et al. J Pharmacol Exp Ther, 2009 Aug. PMID 19458105.
Siglec-8 on human eosinophils and mast cells, and Siglec-F on murine eosinophils, are functionally related inhibitory receptors. Bochner BS. Clin Exp Allergy, 2009 Mar. PMID 19178537.
Natural anti-Siglec autoantibodies mediate potential immunoregulatory mechanisms: implications for the clinical use of intravenous immunoglobulins (IVIg). von Gunten S, et al. Autoimmun Rev, 2008 Jun. PMID 18558361.
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