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Goat Anti-SIRT4 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-SIRT4 Antibody AF1995a
    AF1995a (0.05ug/ml) staining of Human Kidney (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.chemiluminescence.
  • WB - Goat Anti-SIRT4 Antibody AF1995a
    HEK293 overexpressing SIRT4 (RC212226) and probed with AF1361a (mock transfection in first lane), tested by Origene.
  • IHC - Goat Anti-SIRT4 Antibody AF1995a
    AF1995a (2 µg/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y6E7
Other Accession NP_036372, 23409, 75387 (mouse)
Reactivity Human, Rat
Predicted Mouse, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 35188 Da
Additional Information
Gene ID 23409
Other Names NAD-dependent protein deacetylase sirtuin-4 {ECO:0000255|HAMAP-Rule:MF_03161}, 3.5.1.- {ECO:0000255|HAMAP-Rule:MF_03161}, NAD-dependent ADP-ribosyltransferase sirtuin-4 {ECO:0000255|HAMAP-Rule:MF_03161}, 2.4.2.- {ECO:0000255|HAMAP-Rule:MF_03161}, Regulatory protein SIR2 homolog 4 {ECO:0000255|HAMAP-Rule:MF_03161}, SIR2-like protein 4 {ECO:0000255|HAMAP-Rule:MF_03161}, SIRT4 {ECO:0000255|HAMAP-Rule:MF_03161}, SIR2L4
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-SIRT4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SIRT4 {ECO:0000255|HAMAP-Rule:MF_03161}
Synonyms SIR2L4
Function Acts both as NAD-dependent protein ADP-ribosyl transferase and NAD-dependent protein deacetylase. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest. In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor. Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys- 471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis. Down-regulates insulin secretion.
Cellular Location Mitochondrion matrix {ECO:0000255|HAMAP- Rule:MF_03161, ECO:0000269|PubMed:16079181, ECO:0000269|PubMed:16959573, ECO:0000269|PubMed:17715127}
Tissue Location Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level). Widely expressed. Weakly expressed in leukocytes and fetal thymus
Research Areas
Citations (0)

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This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.


Genetic association analysis of 13 nuclear-encoded mitochondrial candidate genes with type II diabetes mellitus: the DAMAGE study. Reiling E, et al. Eur J Hum Genet, 2009 Aug. PMID 19209188.
Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase. Ahuja N, et al. J Biol Chem, 2007 Nov 16. PMID 17715127.
Sirtuin functions in health and disease. Yamamoto H, et al. Mol Endocrinol, 2007 Aug. PMID 17456799.
SIRT4 inhibits glutamate dehydrogenase and opposes the effects of calorie restriction in pancreatic beta cells. Haigis MC, et al. Cell, 2006 Sep 8. PMID 16959573.
The finished DNA sequence of human chromosome 12. Scherer SE, et al. Nature, 2006 Mar 16. PMID 16541075.

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$ 325.00
Cat# AF1995a
(40 western blots)
Availability: 7-10 days
Bulk Size
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