|Application ||WB, E|
|Other Accession||NP_005556, 3937|
|Predicted||Mouse, Rat, Pig, Dog, Cow|
|Calculated MW||60188 Da|
|Other Names||Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76, LCP2|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-SLP76 / LCP2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in T-cell antigen receptor mediated signaling.|
|Tissue Location||Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines Not detected in fibroblast or neuroblastoma cell lines|
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Provided below are standard protocols that you may find useful for product applications.
SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.
Cooperative interactions at the SLP-76 complex are critical for actin polymerization. Barda-Saad M, et al. EMBO J, 2010 Jul 21. PMID 20562827.
SH2 domain containing leukocyte phosphoprotein of 76-kDa (SLP-76) feedback regulation of ZAP-70 microclustering. Liu H, et al. Proc Natl Acad Sci U S A, 2010 Jun 1. PMID 20534575.
In vivo significance of ITK-SLP-76 interaction in cytokine production. Grasis JA, et al. Mol Cell Biol, 2010 Jul. PMID 20457812.
The Bcr-Abl kinase regulates the actin cytoskeleton via a GADS/Slp-76/Nck1 adaptor protein pathway. Preisinger C, et al. Cell Signal, 2010 May. PMID 20079431.
Src homology 2-domain containing leukocyte-specific phosphoprotein of 76 kDa is mandatory for TCR-mediated inside-out signaling, but dispensable for CXCR4-mediated LFA-1 activation, adhesion, and migration of T cells. Horn J, et al. J Immunol, 2009 Nov 1. PMID 19812192.
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