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Goat Anti-SMARCA3 Antibody

Peptide-affinity purified goat antibody

     
  • WB - Goat Anti-SMARCA3 Antibody AF2006a
    AF2006a staining (0.5 µg/ml) of Jurkat lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
    detail
  • WB - Goat Anti-SMARCA3 Antibody AF2006a
    EB05441 staining (0.5µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane). Detected by chemiluminescence.
    detail
  • FC - Goat Anti-SMARCA3 Antibody AF2006a
    EB05441 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q14527
Other Accession NP_620636, 6596, 20585 (mouse)
Reactivity Human
Predicted Mouse, Rat, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 113929 Da
Additional Information
Gene ID 6596
Other Names Helicase-like transcription factor, 3.6.4.-, 6.3.2.-, DNA-binding protein/plasminogen activator inhibitor 1 regulator, HIP116, RING finger protein 80, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3, Sucrose nonfermenting protein 2-like 3, HLTF, HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-SMARCA3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HLTF
Synonyms HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1
Function Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.
Cellular Location Cytoplasm. Nucleus. Nucleus, nucleolus Nucleus, nucleoplasm. Note=Nuclear localization is stimulated by progesterone.
Tissue Location Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
Research Areas
Citations (0)
citation

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Background

This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein.

References

CHFR functions as a ubiquitin ligase for HLTF to regulate its stability and functions. Kim JM, et al. Biochem Biophys Res Commun, 2010 May 14. PMID 20388495.
Role of double-stranded DNA translocase activity of human HLTF in replication of damaged DNA. Blasty谩k A, et al. Mol Cell Biol, 2010 Feb. PMID 19948885.
Biochemical characterisation of the SWI/SNF family member HLTF. MacKay C, et al. Biochem Biophys Res Commun, 2009 Dec 11. PMID 19723507.
Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.
The helicase-like transcription factor is a strong predictor of recurrence in hypopharyngeal but not in laryngeal squamous cell carcinomas. Capouillez A, et al. Histopathology, 2009 Jul. PMID 19614770.

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$ 341.00
Cat# AF2006a
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