|Application ||WB, E|
|Other Accession||NP_115949, 55671, 68734 (mouse), 314388 (rat)|
|Predicted||Mouse, Rat, Dog|
|Calculated MW||95368 Da|
|Other Names||Serine/threonine-protein phosphatase 4 regulatory subunit 3A, SMEK homolog 1, SMEK1, KIAA2010, PP4R3A, PPP4R3A|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-SMEK1 / KIAA2010 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Note=In interphase localized in the cytoplasm and in the nucleus (with higher levels). During metaphase located in pericentriolar regions|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
PP4R4/KIAA1622 forms a novel stable cytosolic complex with phosphoprotein phosphatase 4. Chen GI, et al. J Biol Chem, 2008 Oct 24. PMID 18715871.
Depletion of protein phosphatase 4 in human cells reveals essential roles in centrosome maturation, cell migration and the regulation of Rho GTPases. Martin-Granados C, et al. Int J Biochem Cell Biol, 2008. PMID 18487071.
Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.
Loss of SMEK, a novel, conserved protein, suppresses MEK1 null cell polarity, chemotaxis, and gene expression defects. Mendoza MC, et al. Mol Cell Biol, 2005 Sep. PMID 16107728.
A novel, evolutionarily conserved protein phosphatase complex involved in cisplatin sensitivity. Gingras AC, et al. Mol Cell Proteomics, 2005 Nov. PMID 16085932.
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