Goat Anti-SMEK1 / KIAA2010 Antibody
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q6IN85 |
Other Accession | NP_115949, 55671, 68734 (mouse), 314388 (rat) |
Reactivity | Human |
Predicted | Mouse, Rat, Dog |
Host | Goat |
Clonality | Polyclonal |
Concentration | 100ug/200ul |
Isotype | IgG |
Calculated MW | 95368 Da |
Gene ID | 55671 |
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Other Names | Serine/threonine-protein phosphatase 4 regulatory subunit 3A, SMEK homolog 1, SMEK1, KIAA2010, PP4R3A, PPP4R3A |
Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Goat Anti-SMEK1 / KIAA2010 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PPP4R3A (HGNC:20219) |
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Function | Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140' (gamma-H2AX) generated during DNA replication and required for DNA DSB repair. |
Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus Note=In interphase localized in the cytoplasm and in the nucleus (with higher levels). During metaphase located in pericentriolar regions |
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Provided below are standard protocols that you may find useful for product applications.
References
PP4R4/KIAA1622 forms a novel stable cytosolic complex with phosphoprotein phosphatase 4. Chen GI, et al. J Biol Chem, 2008 Oct 24. PMID 18715871.
Depletion of protein phosphatase 4 in human cells reveals essential roles in centrosome maturation, cell migration and the regulation of Rho GTPases. Martin-Granados C, et al. Int J Biochem Cell Biol, 2008. PMID 18487071.
Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.
Loss of SMEK, a novel, conserved protein, suppresses MEK1 null cell polarity, chemotaxis, and gene expression defects. Mendoza MC, et al. Mol Cell Biol, 2005 Sep. PMID 16107728.
A novel, evolutionarily conserved protein phosphatase complex involved in cisplatin sensitivity. Gingras AC, et al. Mol Cell Proteomics, 2005 Nov. PMID 16085932.
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