|Application ||WB, E|
|Other Accession||NP_055126, 23583|
|Calculated MW||29862 Da|
|Other Names||Single-strand selective monofunctional uracil DNA glycosylase, 3.2.2.-, SMUG1|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-SMUG1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5- formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5- hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
SMUG1 is a glycosylase that removes uracil from single- and double-stranded DNA in nuclear chromatin, thus contributing to base excision repair.
Polymorphisms in the base excision repair pathway and graft-versus-host disease. Arora M, et al. Leukemia, 2010 Aug. PMID 20574454.
Variation within DNA repair pathway genes and risk of multiple sclerosis. Briggs FB, et al. Am J Epidemiol, 2010 Jul 15. PMID 20522537.
Association between genetic variants in the base excision repair pathway and outcomes after hematopoietic cell transplantations. Thyagarajan B, et al. Biol Blood Marrow Transplant, 2010 Aug. PMID 20226869.
Polymorphisms in uracil-processing genes, but not one-carbon nutrients, are associated with altered DNA uracil concentrations in an urban Puerto Rican population. Chanson A, et al. Am J Clin Nutr, 2009 Jun. PMID 19403629.
Opposite-base dependent excision of 5-formyluracil from DNA by hSMUG1. Knaevelsrud I, et al. Int J Radiat Biol, 2009 May. PMID 19365746.
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