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Goat Anti-SNAP25 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-SNAP25 Antibody AF2012a
    AF2012a (0.03 µg/ml) staining of Mouse Brain lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • IHC - Goat Anti-SNAP25 Antibody AF2012a
    AF2012a (2.5 µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P60880
Other Accession NP_570824, 6616
Reactivity Human, Mouse
Predicted Rat, Dog
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 23315 Da
Additional Information
Gene ID 6616
Other Names Synaptosomal-associated protein 25, SNAP-25, Super protein, SUP, Synaptosomal-associated 25 kDa protein, SNAP25, SNAP
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-SNAP25 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SNAP25
Synonyms SNAP
Function t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
Cellular Location Cytoplasm, perinuclear region. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome Note=Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region (By similarity).
Tissue Location Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum
Research Areas
Citations (0)

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Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.


Association Among SNAP-25 Gene DdeI and MnlI Polymorphisms and Hemodynamic Changes During Methylphenidate Use: A Functional Near-Infrared Spectroscopy Study. Oner O, et al. J Atten Disord, 2010 Aug 2. PMID 20679152.
An approach based on a genome-wide association study reveals candidate loci for narcolepsy. Shimada M, et al. Hum Genet, 2010 Oct. PMID 20677014.
An association study between SNAP-25 gene and Attention-Deficit Hyperactivity Disorder. Zhang H, et al. Eur J Paediatr Neurol, 2010 Jul 2. PMID 20599404.
Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
Association between a synaptosomal protein (SNAP-25) gene polymorphism and verbal memory and attention in patients with endogenous psychoses and mentally healthy subjects. Golimbet VE, et al. Neurosci Behav Physiol, 2010 May. PMID 20333500.

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$ 325.00
Cat# AF2012a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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