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Goat Anti-SPHK1 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-SPHK1 Antibody AF2031a
    AF2031a (2 µg/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NYA1
Other Accession NP_892010, 8877, 20698 (mouse), 170897 (rat)
Reactivity Human
Predicted Mouse, Rat, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 42518 Da
Additional Information
Gene ID 8877
Other Names Sphingosine kinase 1, SK 1, SPK 1,, SPHK1, SPHK, SPK
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-SPHK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPHK1
Synonyms SPHK, SPK
Function Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
Cellular Location Cytoplasm. Nucleus. Cell membrane. Note=Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner
Tissue Location Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle
Research Areas
Citations ( 0 )


Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.


SphK1 regulates proinflammatory responses associated with endotoxin and polymicrobial sepsis. Puneet P, et al. Science, 2010 Jun 4. PMID 20522778.
Sphingosine 1-phosphate-metabolizing enzymes control influenza virus propagation and viral cytopathogenicity. Seo YJ, et al. J Virol, 2010 Aug. PMID 20519401.
Sphingosine kinase mediates resistance to the synthetic retinoid N-(4-hydroxyphenyl)retinamide in human ovarian cancer cells. Illuzzi G, et al. J Biol Chem, 2010 Jun 11. PMID 20404323.
New genetic associations detected in a host response study to hepatitis B vaccine. Davila S, et al. Genes Immun, 2010 Apr. PMID 20237496.
CXCL4-induced monocyte survival, cytokine expression, and oxygen radical formation is regulated by sphingosine kinase 1. Kasper B, et al. Eur J Immunol, 2010 Apr. PMID 20104488.

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$ 325.00
Cat# AF2031a
(40 western blots)
Availability: 7-10 days
Bulk Size
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